The germinal epithelium suddenly ends with begin of the rete testis, forming a kind of valve to prevent the backflow of spermatozoa. Following dissection and routine histological preparation, histopathological and stereological investigations were carried out. A systematic evaluation approach will yield the most information. A brief overview of the histological features of the testis useful for all healthcare students. The body weights, food consumption, hematology, serum biochemistry, organ weight, and histopathological examination were compared between rats fed on gm soybean and those. This study was conducted to evaluate the effect of rj on histopathological alterations of the testicular tissue in streptozotocin. Nanoparticles ag nps have recently received much attention for their possible applications in biotechnology and biomedical. The seminiferous tubules start and end at the rete testis.
However, formatting rules can vary widely between applications and fields of interest or study. Histological and histopathological evaluation of the testis by lonnie dee russell, 1990, cache river press edition, in english 1st ed. Histological and histopathological evaluation of the testis lonnie d. However, little is known about the toxicity in reproductive organs of animal model following exposure to nanoparticles. Histology of the testis, epididymis and spermatogenesis. Basic positive calretinin, ema, podoplanin, mesothelin and negative cea, berep4 immunohistochemical ihc marker reactions were determined. Incidence of spermatogenic suppression, leydig cell atrophy and vacuolation of sertoli cells were most prominent in testicular sections from primiphos methyl treated animal testis than in animals of the other. The treated groups were received 10, 50 or 100 iukg bw of hcg. Profile of secondary sex organ changes with androgen. In a retrospective study we analyzed clinical data including diagnostic procedures, intraoperative findings, final diagnosis, treatment modality, and outcome of patients with impalpable testis who underwent surgery from january 2010 until december 2015. Read histological and histopathological evaluation of the. Historical data from our lab showed that the left testis weighed 2.
Then you can start reading kindle books on your smartphone, tablet, or computer no kindle device required. Ten normal testes from 7weekold wistar rats were ased for the study. Histological and histopathological evaluation of the testis. No injection was given to the rats in the control group. Bats show a diverse range of reproductive strategies, although their reproductive skills are directly related to individual body condition, which makes the availability of food a determining factor for the onset of the reproductive cycle in these animals, the bat sturnira lilium e. The aim of this study is to present our treatment protocol for impalpable testis. Between june 1998 and march 2009, 29 patients underwent surgery for leydig cell tumor of the testis in our cancer network. Russell and others published histological and histopathological evaluation of the testis find, read and cite. Read feasible etiology of vanishing testis regarding disturbance of testicular development. The histopathological effects of an electromagnetic field. Recommended approaches for the evaluation of testicular.
Evaluation of testis hormonal and histopathological. The histopathological evaluation of a testicular biopsy is a very important part of the diagnostic workup of andrological patients with azoospermia or severe oligozoospermia as well as men at risk for germ cell neoplasia in situ. Russell and others published histological and histopathological evaluation of the testis find, read and cite all the research you need on researchgate. Biochemical and histopathological studies on female and.
Histopathological and immunohistochemical evaluation of testicular nubbins, international journal of urology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Histological examination by opening the abdominal cavity, the testes were removed in order to determine histomorphometric and histopathologic changes. This is one of the most important contributions to the field of testicular histology and histopathology that i have seen, and one. Read histological and histopathological evaluation of the testis ebooks online. Evaluation of the cell population of the seminiferous. Vanished testes are identified in up to 35% of cases of nonpalpable maldescended testes. This tumor is extremely rare in adult men, but a juvenile type accounts for 6% of testis tumor in childhood. Ii, and iii lesions may vary, and in lymphomas different types of histological lesions may be variably represented, it is quite unlikely that it could solely account for the observed variability. Histopathological evaluation provides one of the most sensitive end points for detecting the effects of toxicants on the testis, and is used routinely in the assessment of male reproductive toxicity. The testicular biopsy was labeled mixed if more than one pattern was seen in the same biopsy from the same side and discordant if the right and left testicular biopsies showed different patterns. Histological and histopathological evaluation of the. Learn vocabulary, terms, and more with flashcards, games, and other study tools. International journal of shahid sadoughi university of.
Evaluation of testicular toxicity in safety evaluation studies. The aim of this study was to evaluate the effects of a diet containing gm soybean meal rur on male and female wistar rats. Histological and histopathological evaluation of the testis 286 by lonnie dee russell, et al, robert ettlin, eric d. Bilateral histological evaluation of exocrine testicular. Median histological score for right and left testis was 9. This book, the first to describe how the testis is evaluated in research and toxicology testing settings, is a resource for individuals who wish to perform a systematic evaluation of the testis. Histopathological evaluation of apoptosis in cancer. Rat testis excised at 0, 12, 24, 36, and 48 hours after death showed progressive gross and histopathological changes.
Stereological and histopathological evaluation of ovary. Clegg cache river press, clearwater, florida, 1990. The book begins with a description of normal testis structure. Histological and histopathological evaluation of the testis evaluation of the testis. Recently, transplantation of mouse donor spermatogonial stem cells from a fertile testis to an infertile recipient mouse testis was described1,2. One more thing noted was the histological uniformity within the same testis as well as between right and left testes. After spontaneous delivery, male offspring were obtained. The biopsies were examined for histological uniformity within the same testis and between right and left testes. Histopathological testicular studies and research into the correlations between histological and echotexture data are limited, however vital et al.
Granulosa cell tumors of the testis are microscopically identical to granulosa cell tumors of the ovary. Geoffroy, 1810 belongs to the phyllostomidae family and presents essentially frugivorous. The biopsy is also often the only way for infertile men to become biological fathers. Pathologic evaluation is generally performed on the entire testis rather than on a biopsy sample. In recent years histological and physiological studies have increased in the evaluation of the effects of electromagnetic fields on human health kang et al. It is important for the pathologist to suspect dili when the histological changes are unusual or out of synchronicity with the patients history. The aim of this study is to investigate the histological findings of testicular nubbins in patients with nonpalpable testis and to question etiology and surgical indications for vanishing testis. Histopathological patterns of testicular biopsy in male. Germcell cancer of the testis is relatively uncommon, accounting for about 1% of all cancers in men. Septa which emanate from this capsule to subdivide the testis into about 250 incomplete lobules. Histological sections of testes, liver and kidneys were examined and histopathological changes and quantitative estimates were recorded. Histological investigations showed that the epididymis had no discrete compartments. Effects of human chorionic gonadotropin on the testis of. The macromorphometric evaluation of the right and left testes did not reveal significant differences.
In this study, we applied the immunohistologic procedure using antiproliferating cell nuclear antigen pcna monoclonal antibody for staging germ cells in seminiferous tubules, and examined whether it was useful for histopathological screening of the testis. At the end of the 4th week, ovary and uterine horn samples were removed. Histological evaluation of the testicular nubbins in. Histological and histopathological evaluation of the testis evaluation of the testis bibliographic information language. This study therefore, tried to examine the effects of nanoparticles with a diameter range of 520 nm on the histology of the testis of wistar rats. Furthermore, histopathological evaluation of the testis is a difficult and timeconsuming task due to the organization complexity of testicular tissue reflecting the multiple and intricate steps of spermatogenesis, particularly in eutherian mammals 23. The spermatozoa are made in the semiferous tubules of the testes.
Pdf histological methods for the evaluation of the testis. Application of pcna immunohistochemistry for pathological. Read zinc therapy improves deleterious effects of chronic copper administration on mice testes. Results a significant reduction in the testosterone, fsh, and lh levels were observed in t1dm, t2dm, and also in. Testis weight decreased throughout the time course. Histological and histopathological evaluation of the testes, russell ld, ettlin r, sinha hikim ap, clegg ed eds.
Reproductive biology and histopathological of orechromis. Histological and anatomical study of the white rooster of. Roundup ready became the most cultivated genetically modified gm crop in the world. Evaluation of histopathologic and histomorphometric. Profile of secondary sex organ changes with androgen receptor antagonism. Reevaluation of histological diagnoses of malignant.
Hekimgil m1, altay b, yakut bd, soydan s, ozyurt c, killi r. The impact of histopathologic diagnosis on the proper. If more than one histological pattern was noted in the same testicular biopsy it was. The septa converge towards the midline of the posterior border, meeting along a thickening of the tunica albuginea called the mediastinum testis. It has adverse effects on male reproductive function. Four patients with sub5 millimetre lesions underwent testis sparing surgery and 25 were treated with radical orchidectomy. Enter your mobile number or email address below and well send you a link to download the free kindle app. Histological evaluation of the human testisapproaches to.
Histological staining was used to check abnormal patterns of testicular morphology, the diameter of seminiferous tubules, testicular diameter, and germinal layer thickness. The donor cells established spermatogenesis in the. Indeed, there are a number of methodological and technical factors that may influence the. Comparative histopathological evaluation of permethrin.